General Information

  • ID:  hor003129
  • Uniprot ID:  Q9VIQ0
  • Protein name:  sNPF-associated peptide
  • Gene name:  sNPF
  • Organism:  Drosophila melanogaster (Fruit fly)
  • Family:  NPY family
  • Source:  Animal
  • Expression:  Expressed throughout development from embryo to adult. |Stage 17 embryos show expression in the two brain hemispheres (neural cells located in the dorsal posterior region), the connected ventral ganglion (pairs of neural cells along the ventral midline) a
  • Disease:  NA
  • Comments:  NA
  • Taxonomy:  melanogaster subgroup, melanogaster group, Sophophora (subgenus), Drosophila (genus), Drosophilini (tribe), Drosophilinae (subfamily), Drosophilidae (family), Ephydroidea (superfamily), Acalyptratae, Schizophora, Cyclorrhapha, Eremoneura, Muscomorpha (infraorder), Brachycera (suborder), Diptera (order), Endopterygota (cohort), Neoptera (infraclass), Pterygota (subclass), Dicondylia, Insecta (class), Hexapoda (subphylum), Pancrustacea, Mandibulata, Arthropoda (phylum), Panarthropoda, Ecdysozoa, Protostomia, Bilateria, Eumetazoa, Metazoa (kingdom), Opisthokonta, Eukaryota (superkingdom), cellular organisms
  • GO MF:  GO:0005184 neuropeptide hormone activity; GO:0048018 receptor ligand activity; GO:0071855 neuropeptide receptor binding
  • GO BP:  GO:0007218 neuropeptide signaling pathway; GO:0007623 circadian rhythm; GO:0008340 determination of adult lifespan; GO:0008343 adult feeding behavior; GO:0010906 regulation of glucose metabolic process; GO:0030536 larval feeding behavior; GO:0032095 regulation of response to food; GO:0035264 multicellular organism growth; GO:0040014 regulation of multicellular organism growth; GO:0040018 positive regulation of multicellular organism growth; GO:0045793 positive regulation of cell size; GO:0070374 positive regulation of ERK1 and ERK2 cascade; GO:0090062 regulation of trehalose metabolic process
  • GO CC:  GO:0005576 extracellular region; GO:0005615 extracellular space; GO:0030424 axon; GO:0043025 neuronal cell body

Sequence Information

  • Sequence:  SDPDMLNSIVE
  • Length:  11(80-90)
  • Propeptide:  MFHLKRELSQGCALALICLVSLQMQQPAQAEVSSAQGTPLSNLYDNLLQREYAGPVVFPNHQVERKAQRSPSLRLRFGRSDPDMLNSIVEKRWFGDVNQKPIRSPSLRLRFGRRDPSLPQMRRTAYDDLLERELTLNSQQQQQQLGTEPDSDLGADYDGLYERVVRKPQRLRWGRSVPQFEANNADNEQIERSQWYNSLLNSDKMRRMLVALQQQYEIPENVASYANDEDTDTDLNNDTSEFQREVRKPMRLRWG
  • Signal peptide:  MFHLKRELSQGCALALICLVSLQMQQPAQA
  • Modification:  NA
  • Glycosylation:  NA
  • Mutagenesis:  NA

Activity

  • Function:  Plays a role in controlling food intake and regulating body size.
  • Mechanism:  Flies overexpressing sNPF are heavier and bigger than wild-type. But loss-of-function flies are not smaller than wild-type.
  • Cross BBB:  NA
  • Target:  sNPF-R
  • Target Unid:  Q9VW75
  • IC50: NA
  • EC50: NA
  • ED50: NA
  • kd: NA
  • Half life: NA

Structure

  • Disulfide bond:  NA
  • Structure ID:  AF-Q9SIZ9-F1(AlphaFold_DB_ID)

  • Structure: (PDB_ID-from https://www.rcsb.org/; AlphaFold_DB_ID-from https://alphafold.ebi.ac.uk/; hordbxxxxxx_AF2.pdb was predicted structure by AlphaFold2; hordbxxxxxx_ESM.pdb was predicted structure by ESMFold)
    •    AF-Q9SIZ9-F1.pdbhor003129_AF2.pdbhor003129_ESM.pdb

Physical Information

Mass: 139788 Formula: C50H82N12O21S
Absent amino acids: ACFGHKQRTWY Common amino acids: DS
pI: 3.38 Basic residues: 0
Polar residues: 3 Hydrophobic residues: 3
Hydrophobicity: -25.45 Boman Index: -2146
Half-Life: 1.9 hour Half-Life Yeast: >20 hour
Half-Life E.Coli: >10 hour Aliphatic Index 97.27
Instability Index: -630 Extinction Coefficient cystines: 0
Absorbance 280nm: 0

Literature

  • PubMed ID:  12171930
  • Title:  Peptidomics of the Larval Drosophila Melanogaster Central Nervous System.
  • PubMed ID:  15385546
  • Title:  Drosophila Short Neuropeptide F Regulates Food Intake and Body Size